WT BMDM M2 vs RON TK- BMDM M2. WT BMDM M2 vs RON TK- BMDM M2

Introduction: HGFL-Ron signaling is augmented in human breast cancer and is associated with poor overall prognosis. Here, we investigate the role of HGFL-Ron signaling in RON-modulated murine macrophages through RNA-sequencing of bone marrow-derived macrophages from FVB WT or FVB RON tyrosine kinase -/- mice. BMDM of each genotype M2-polarized via 72 hour treatment with IL-4 were submitted for transcriptomic characterization on the Illumina HiSeq 2500. High quality reads were aligned to the mm10 genome and quantified to generate RPKM Overall design: Transcriptome profiles of RON-proficient and RON-deficient BMDM M2 cells through RNA-sequencing