Figure_3_Analysisperiodontaltissues.csv

Figure 3- Analysis of periodontal tissues of infected or sham-infected mice subjected to the control or HF diets. (A) Representative images of hemimaxillae of mice from different experimental groups. (B) Analysis of alveolar bone loss after 12 weeks with diet and 4 weeks after oral inoculation of A. actinomycetemcomitans. The values (mean ± S.E.M) were obtained from 5 - 7 animals at each point in two independent experiments, with a total of 35 mice. *p<0.05 when compared to control. One-way ANOVA followed by Newman-Keuls. (C) Heat-map representing differences on the oral microbiota analysis between mice fed with the control diet infected or non-infected with Aa, and non-infected mice fed with the HF diet. Groups: C — Sham-infected group on the control diet. Aa — Group on the control diet, infected with A. actinomycetemcomitans HF — Group on the HF diet, non-infected. N=5. *p<0.05 Aa compared to HF; #p<0.05 C compared to Aa; +p<0.05 C compared to HF. A two-way ANOVA — Bonferroni multiple comparison test was performed. The values (mean ± S.E.M) are representative of two independent experiments