Assembled Illumina Short Reads
收藏figshare.com2023-06-01 更新2025-01-22 收录
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Assembled Illumina short reads from human oral cavity samples processed using the state-of-the-art DNA extraction strategies for shotgun metagenomics described in the following study:https://doi.org/10.1101/2021.03.03.433801These two metagenomes are from the same individual's oral samples and generated from (1) the sample used for long-read sequencing of the same material to test HMW DNA extraction method 01 in our study (here named ORAL_ILLUMINA_METHOD_01_REPL_02_ASSEMBLED) and (2) the sample used for 16S rRNA gene amplicon sequencing (here named ORAL_ILLUMINA_TD_ASSEMBLED.db).These contigs databases here includes sequences longer than 2,500 and which includes gene calls, HMM hits for single-copy core genes, and gene functions. Visit this page to learn what you can do with a contigs database:https://merenlab.org/m/contigs-dbPS: we also have a set of anvi'o contigs databases for unassembled and assembled long-reads generated from the same individual in different data packs. For their DOIs, please see the Data Availability section in our study.
本数据集整合了来自人类口腔样本的Illumina短读段,这些读段经过最先进的DNA提取策略处理,具体方法详见以下研究:https://doi.org/10.1101/2021.03.03.433801。该研究中的两个宏基因组数据集均源自同一个体的口腔样本,其中(1)一个样本用于本研究中长读长测序材料的长链DNA提取方法01(以下简称ORAL_ILLUMINA_METHOD_01_REPL_02_ASSEMBLED)的测试;(2)另一个样本用于16S rRNA基因扩增子测序(以下简称ORAL_ILLUMINA_TD_ASSEMBLED.db)。此处所包含的contigs数据库涵盖了长度超过2,500碱基的序列,并包含基因注释、针对单拷贝核心基因的HMM搜索命中以及基因功能信息。欲了解contigs数据库的用途,请访问此页面:https://merenlab.org/m/contigs-db。此外,我们还拥有一套anvi'o contigs数据库,用于存储不同数据包中同一个体未组装及组装的长读长数据。相关DOI信息请参阅本研究的数据可用性部分。
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