Specific ablation of CD4+ T-cells promotes neonatal heart regeneration in mice. Mus musculus

In order to understand the heterogeneity of different T-cell subsets of the regenerating and non-regenerating hearts in response to myocardial injury, we performed droplet-based genome-wide single-cell transcriptomic profiling (scRNA-seq). At day 7 after injury, we purified CD45+CD3+ T-cells from the hearts of ICR mice that underwent CI at P3 and P8 by flow cytometry; and captured the transcriptome of about ~1,123 and ~2,167 cells by scRNA-seq, respectively.