Type-I nNOS neurons orchestrate cortical neural activity and vasomotion

It is unknown how the brain orchestrates the coordination of global neural and vascular dynamics. We sought to uncover the role of a sparse but unusual population of genetically-distinct interneurons known as type-I nNOS neurons, using a novel pharmacological strategy to unilaterally ablate these neurons from the somatosensory cortex of mice. Region-specific ablation produced changes in both neural activity and vascular dynamics, decreased power in the delta-band of the local field potential, reduced sustained vascular responses to prolonged sensory stimulation, and abolished the post-stimulus undershoot in cerebral blood volume. Coherence between the left and right somatosensory cortex gamma-band power envelope and blood volume at ultra-low frequencies was decreased, suggesting that type-1 nNOS neurons integrate long-range coordination of brain signals. Lastly, we observed decreases in the amplitude of resting-state blood volume oscillations and decreased vasomotion following the ablat..., , , # Type-I nNOS neurons orchestrate cortical neural activity and vasomotion

This document outlines the steps necessary to generate the figures for the manuscript **Type-I nNOS neurons orchestrate cortical neural activity and vasomotion** by Kevin L Turner, Dakota F Brockway, Md Shakhawat Hossain, Keith R Griffith, Denver I Greenawalt, Qingguang Zhang, Kyle W Gheres, Nicole A Crowley, and Patrick J Drew.

---

## Generating the figures

---

The code in this repository generates all main figures, supplemental information, and statistics. It also contains the analysis code for all pre-processing steps.

Begin by downloading the code repository by clicking the green **Code** button at the top of the page and selecting **Download ZIP**.

* Code repository location: [https://github.com/KL-Turner/Turner-eLife2025](https://github.com/KL-Turner/Turner-eLife2025)


Next, the d...,