Chemical-induced cardiac reprogramming in vivo
收藏干细胞与再生医学数据中心2021-09-08 更新2024-03-06 收录
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1. Immunofluorescence staining of cryosections of the heart was used to determine the occurrence of in situ cardiac transdifferentiation, and the size and number of cardiomyocytes derived from the transdifferentiation were observed.The samples were divided into two groups: the control group and the small molecule compound group.
2. Use echocardiography to determine the degree of injury and repair of cardiac function after injury.The samples were divided into two groups: the control group and the small molecule compound group.
3. Masson trichrome staining was used to determine the area of cardiac fibrosis and to observe the changes of the area of cardiac fibrosis after transdifferentiation. The samples were divided into two groups: the control group and the small molecule compound group.
4. Patch clamp was used to detect the action potential of single cardiomyocytes after cardiac digestion, and the expression of CICMs action potential was detected.
提供机构：
同济大学
创建时间：
2021-09-08
