Analysis of microRNA Expression Characteristics Related to Low Temperature Stress in Chewing Cane

[Objectives] To explore microRNA expression characteristics related to low temperature stress in chewing cane. [Methods] The research on miRNA under abiotic stress of sugarcane at home and abroad mainly focused on the types and regulation of miRNA under cold, heat, drought, high salt, and mechanical stress. However, there are few studies on miRNA under low temperature stress in chewing cane. The target genes of miR394 and miR825 in chewing cane were predicted and functionally analyzed by bioinformatics technology. [Results] The results showed that the target genes of miR394 and miR825 were mainly members of the WRKY transcription factor family, involved in plant growth, development and stress resistance. Real-time fluorescence quantitative PCR analyzed the expression characteristics of target miRNA in different tissues of chewing cane at different periods of low temperature stress. [Conclusions] The results showed that the expression of chewing cane miR394 and miR408 had temporal and spatial specificity and tissue specificity, both of which could respond to low temperature stress with significant differential expression.