Gene Expression Profile in Caco-2 Cells Treated With 13-HPODE.

Lipid peroxides (LOOH) have been implicated in the pathology of diverse diseases, such as gut disease, cardiovascular disease, and cancer. Peroxidized lipids abound in processed food. Recently, RNA Sequencing (RNA-seq) has been widely used to profile gene expression. To characterize gene expression and pathway dysregulation upon exposure to peroxidized linoleic acid, we incubated human intestinal epithelial cells (Caco-2) with 100uM of 13-hydroperoxyoctadecadienoic acid (13-HPODE), linoleic acid (LA) or hydrogen peroxide (H2O2) for 24h. Total RNA was extracted for library preparation and Illumina HiSeq sequencing. This study provides insights into mechanisms by which 13-HPODE alters cellular and metabolic processes, possible involvement in mitochondrial dysfunction-related disorders and proposes potential therapeutic strategies to treat LOOH-related pathologies.