Liver-specific matrin-3 knockout alters the hepatic transcriptome of chow diet-fed mice [RNA-seq]

Matrin-3 is an RNA-binding protein involved in the pathogenesis of human diseases. Here we examined the hepatic transcriptome of chow-diet fed mice. Bulk RNA-seq and bioinformatics analysis identified 646 and 145 differentially expressed genes (Padj < 0.05), respectively in the livers of female and male matrin-3 LKO mice. Enrichr analysis of these DEGs revealed several common Hallmark terms including cholesterol homeostasis, fatty acid metabolism, xenobiotic metabolism, and epithelial mesenchymal transition between female and male mice. Our data demonstrated that liver-specific matrin-3 deficiency re-programs the hepatic transcriptome in the liver of chow diet-fed mice. Matrin-3 floxed mice were bred with Albumin-Cre mice (The Jackson Laboratory, #003574) to produce liver-specific matrin-3 knockout mice (LKO). Mice were housed on a normal 12/12 light-dark cycle. Mice had ad libitum access to a normal chow diet (Envigo, cat# 2016) and water. At 16 weeks of age, mice were euthanized for tissue collection. Total RNAs were extracted from mouse tissues after homogenization using TRIzol Reagent (Thermo Fisher, 15596026) according to the manufacturer’s instructions. For RNA sequencing by Novogene, total RNA quality was examined using Agilent 2100 Bioanalyzer. The RNA Integrity Number of each RNA sample is above 8.5. A total amount of 1 µg RNA per sample was used for library construction. Total eight libraries were constructed; one library was produced from two liver tissues; and each group of mice has two library replicates.