Dataset for "Predicting Ligand-Free Cell Attachment on Next Generation Cellulose-Chitosan Hydrogels"

There is a growing appreciation that engineered biointerfaces can regulate cell behaviors, or functions. Most systems aim to mimic the cell-friendly extracellular matrix environment and incorporate protein ligands; however, the understanding of how a ligand-free system can achieve this is limited. Cell scaffold materials comprised of interfused chitosan–cellulose hydrogels promote cell attachment in ligand-free systems, and these data were used to demonstrate the role of cellulose molecular weight (MW) and chitosan content and MW in controlling material properties and thus regulating cell attachment.

Included are the data used for determining gel shear moduli; data used in cell attachment regression models and for determining average cell attachment; combined DAPI and FITC images of 24 hour cell morphology studies; data used for determining median cell aspect ratio, cell aspect ratio distribution, and median cell area; UV-vis adsorbance data; confocal microscopy data used for calculating chitosan penetration into cellulose gels; FTIR spectra data and fitted curves; scanning probe microscopy data used for determining gel surface roughness and capacitive coupling; and measurements of chitosan MW and gel surface zeta-potential.

日益凸显的是，经过设计的生物界面能够调控细胞行为或功能。多数系统旨在模拟细胞友好的细胞外基质环境并整合蛋白配体；然而，对于无配体系统如何实现此功能的理解尚显局限。由互穿壳聚糖-纤维素水凝胶构成的细胞支架材料在无配体系统中促进了细胞的附着，这些数据被用于展示纤维素分子量（MW）和壳聚糖含量及分子量在控制材料性质及调节细胞附着方面的作用。包括用于确定凝胶剪切模量的数据；用于细胞附着回归模型和确定平均细胞附着的资料；24小时细胞形态研究的DAPI和FITC结合图像；用于确定细胞长宽比中位数、细胞长宽比分布和细胞面积中位数的数据；紫外-可见光吸附数据；用于计算壳聚糖穿透纤维素凝胶的共聚焦显微镜数据；傅里叶变换红外光谱数据和拟合曲线；用于确定凝胶表面粗糙度和电容耦合的扫描探针显微镜数据；以及壳聚糖分子量凝胶表面zeta电位的测量。