C-X-C chemokine receptor 4 ablation-driven transcriptional changes in monocyte-derived macrophage in response to lipopolysaccharide

Purpose: Next-Generation Sequencing (NGS) RNA was subjected to analysis genome-wide expression. The goals of this study are to identify differentially expressed genes in CXCR4 knockout macrophages compared to CXCR4-expressed macrophages. Methods: Total RNA of macrophage transfected with CXCR4 siRNA (MFsiCXCR4) or siRNA negative control (MFsiNC), then, stimulated with 100ng/ml lipopolysaccharide (LPS) for 12 hours. Preparation of library and sequencing of transcriptome was performed by Illumina NovaSeq 6000. Results: A large number of differentially expressed genes were screened by high-throughput sequencing at the genome-wide level using RNA-Seq technology. Loss of CXCR4 in bone-marrow-derived macrophages results in attenuated pro-inflammatory cytokine and decorin production under LPS stimulation compared to control macrophages. Conclusions: Our study reveals that CXCR4 orchestrated the pro-inflammatory cytokine and decorin production in macrophages. Overall design: Gene expression profiling from macrophage transfected with CXCR4 siRNA (MFsiCXCR4) or siRNA negative control (MFsiNC) after LPS (100ng/ml, 12hours) treatment.