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Single cell sequencing of mouse gamma-delta T cells

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Mouse gamma-delta T cells were sorted from adult C57Bl/6 mouse spleen and lymph nodes and cells were subjected to scRNA-seq Single cell suspensions from pooled spleens and lymph nodes were stained with viability dye, CD45, CD3 and gdTCR. Cells were gated on viable, CD45+CD3+gdTCR+ cells.

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