Deep Transcriptome Analysis for 9 tissues of Sophora flavescens

More than 200 million fastq format reads were generated by Illumina's next-generation sequencing approach using nine types of tissue from Sophora flavescens, followed by CLC de novo assembly, ultimately yielding 83325 contigs in total. By mapping the reads back to the contigs, RPKM values were calculated to demonstrate gene expression levels, and overrepresented gene ontology terms were evaluated using Fisher's exact test. In search for the putative genes relevant to essential metabolic pathways, all 1350 unique enzyme commision numbers were used to map pathways against KEGG.