Analysis of transcriptional responses of Saccharomyces cerevisiae to mannitol and 4-deoxy-L-erythro-5-hexoseulose uronate (DEH).

We constructed S. cerevisiae BY_DEH+ strain which is able to assimilate both 4-deoxy-L-erythro-5-hexoseulose uronate (DEH, a monouronic acid produced by digestion of alginate with exo-type alginate lyase) and mannitol from BY4742 strain and improved its ability to assimilate DEH through an adaptive evolution (Matsuoka et al. Sci. Rep. 2017, 7, 4206). To examine transcriptional responses of the yeast to DEH and mannitol, gene expressions of the evolved strain (BY_DEH++ strain) in DEH medium, mannitol medium, and glucose medum were analyzed. For revealing the mechanisms underlying the adaptive evolution, gene expressions of both BY_DEH+ strain and BY_DEH++ strain in both DEH medium and glucose medium were measured. Overall design: Cap analysis of gene expression (CAGE) of BY_DEH+ strain cultured in DEH medium and glucose medium and BY_DEH++ strain cultured in DEH medium, mannitol medium, and glucose medium.