Transcriptome - Pirum gemmata
收藏资源简介:
- clean.fasta: Assembly cleaned from cross-contamination of other species multiplexed (Corallochytrium limacisporum Hawaii, Abeoforma whisleri and Parvularia atlantis [Nuclearia sp ATCC 50694]) after applying CroCo V0.1.1 (Simion et al. in prep).<br><br>- Assembly in-home BGI to obtain Unigene (contigs)- ORF prediction using TransDecoder (Trinity plugin) and PfamScan to obtain coding sequences (CDS files) from transcripts (mRNA)- Protein translations are included (pep)<br><strong>Guifré Torruella, Alex de Mendoza, Xavier Grau-Bové, Meritxell Antó, </strong>Mark A. Chaplin<strong>, Javier del Campo, </strong>Laura Eme, Gregorio Pérez-Cordón, Christopher M. Whipps, Krista M. Nichols, Richard Paley, Andrew J. Roger, Ariadna Sitjà-Bobadilla, Stuart Donachie,<strong> and Iñaki Ruiz-Trillo. </strong>(2015) <em>Phylogenomics reveals convergent evolution of lifestyles in close relatives of animals and fungi.</em> <strong><em>Current Biology </em></strong>25(18):2404-10.<br>www.multicellgenome.com
- clean.fasta:经CroCo V0.1.1(Simion等,待发表)处理后,去除了与多重测序的其他物种(Corallochytrium limacisporum Hawaii、Abeoforma whisleri及Parvularia atlantis [Nuclearia sp ATCC 50694])的交叉污染的基因组组装序列文件。<br><br>- 由BGI内部完成组装以获取Unigene(重叠群):使用TransDecoder(Trinity插件)与PfamScan进行开放阅读框预测,从转录本(mRNA)中提取编码序列(CDS文件),并附带蛋白质翻译结果(pep文件)。<br><strong>Guifré Torruella、Alex de Mendoza、Xavier Grau-Bové、Meritxell Antó、Mark A. Chaplin、Javier del Campo、Laura Eme、Gregorio Pérez-Cordón、Christopher M. Whipps、Krista M. Nichols、Richard Paley、Andrew J. Roger、Ariadna Sitjà-Bobadilla、Stuart Donachie以及Iñaki Ruiz-Trillo</strong>(2015年)《系统发育基因组学揭示动物与真菌近亲的生活方式趋同演化》,发表于<strong><em>当代生物学(Current Biology)</em></strong> 25(18):2404-10。<br>www.multicellgenome.com




