Fluorescence polarization of Str enolase interacting with IAF-Pg S741C-hPgn.

Fluorescently-labeled hPgn was used at a final concentration of 140 nM. Str enolase, when present, was 0.057 mM. The temperature was kept at 20°C. The Eclipse fluorometer settings were: slits, 5 nm excitation and emission wavelengths were 470 nm and 516 nm respectively. The G factor was determined for each measurement. All measurements were done five times; the spread between the calculated polarization values was never more than ±0.01. The entire experiment was repeated one time.