aamplicon size: 7.5 kb. bexpected amplicon size: 1.8 kb; non-homologously recombined DNA is not expected to amplify. cexpected amplicon size: 0.7 kb; wildtype allele is not expected to amplify. dexpec
(A) RT-PCR primers for verifying AKAP13 gene-trap splicing events are listed. The primer locations and mutant line verified are indicated. The size of the RT-PCR product is given in base pairs (bp). (
