Regulation of chromatin accessibility in hypoxia condition

Hypoxia leads to significant changes at the histone modification marks, including increasing methylation of H3K4, H3K9 and H3K27. However, the overall effect on chromatin accessibility is not fully understood. Here, we employed an ATACseq method on PFA-fixed mouse glioma cell line GL261to test the genomewide chromatin accessibility changes in response to two hypoxic conditions: moderate hypoxia (1% O2) and severe hypoxia (<0.1% O2). Examinnation of chromatin accessibility with ATACseq in mouse glioma cells line (GL261) in response to severe (<0.1% O2) or mild hypoxia (1% O2) compare to normoxia conditions on PFA fixed cells.