<i>dPNUTS</i> mutants deregulate the expression of the majority of genes in 1^st instar larvae, including highly expressed genes involved in cellular metabolism and proliferative growth.

A) MA plot of RNA-Seq data in which the log2 of the ratio of abundance of each transcript between dPNUTS13B (13B) mutant and w1118 (WT) control (M) is plotted against the log2 geometric average of abundance (FPKM) in both conditions (A). Transcripts with an FPKM of less than 0.25, which are a source of noise in these plots, are not shown for clarity. Transcripts that are differentially expressed (DE) by <0.67 or >1.5 fold are shown in grey; unaffected transcripts are shown in white. Loci corresponding to enriched Gene Ontology (GO) terms amongst the differentially expressed genes relative to the entire genome are highlighted in yellow (enriched amongst overexpressed genes) or red (enriched amongst underexpressed genes). Log2 median expression for genes expressed in WT and 13B is indicated with a dashed line. Log2 median expression for genes belonging to GO categories is given in the legend. A complete list of GO categories is provided in the Supplementary information. B) Expression levels of the indicated genes in dPNUTS9B/dPNUTS9B and dPNUTS13B/dPNUTS13B mutant larvae, relative to control (w1118) larvae, determined by qRT-PCR. Error bars represent the SE (n≥3 biological replicates). The GO categories to which the genes belong are shown at the top.