glioma-associated mesenchymal stem cell (GA-MSCs) transcriptome chip data

GA-MSCs extracted from glioblastoma specimens classified as WHO grade IV (confirmed by pathological examination) were cultured in T25 culture flasks. Once the cell density reached approximately 70%-80%, the cells were treated with either DMEM (control condition) or GSC-CM (experimental condition). Each condition was tested in triplicate. After three days of incubation, total RNA was extracted using the TRIzol reagent (TAKARA, Japan). RNA quality was assessed using a Nanodrop 2000 and an Agilent Bioanalyzer 2100, and only samples meeting quality control criteria were included in the microarray analysis. The RNA samples were poly-A-tailed, biotin-labeled, and analyzed using the Affymetrix GN-GeneChip Human Clariom D Array 2.0 (Affymetrix, USA). The experiment analyzed a total of six samples, with three replicates in both the experimental group and the control group.