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Images of H4K8ac Immunostain in IMR90 Cells

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NIAID Data Ecosystem2026-03-14 收录
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https://zenodo.org/record/7677818
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Files are two-channel images at 64 unique xy positions showing the fluorescence intensity of a DRAQ5 DNA stain (c1) and H4K8ac immunostain with an Alexa488 secondary (c2). Imaging was performed using a Nikon Ti2-E inverted microscope equipped with a Yokogawa CSU-W1 confocal spinning disk unit and a Zyla 2.3 PLUS sCMOS camera. We used 488 nm and 647 nm laser lines paired with 525/36 (MVI, 77074803), and 705/72 (MVI, 77074329) emission filters, respectively. Samples were imaged through a 1.15 NA CFI Apo LWD Lambda S 40X water immersion objective lens (Nikon MRD77410). The imagining system was controlled by NIS-Elements AR software.
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2023-02-26
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