Gene expression profile at single nucleus level of the generated neural concentroids with or without vascularization

We generated homogeneous neural constructs called neural concentroids. We used single nucleus RNA sequencing (snRNA-seq) to analyze the diversity of brain cell types in the neural concentroids with or without the incorporation of hiPSC-derived endothelial cells as an effect of vascularization Overall design: Cell nuclei were isolated by in-house protocols and analyzed using snRNAseq.