Contact- and water-mediated interactions with an allelopathic macroalga drive distinct coral microbiome and metabolome

Manipulative field experiment in Mo'orea (French Polynesia) testing the relative influence of contact vs. water-mediated interactions on coral microbiome and metabolome with the allelopathic alga Dictyota bartayresiana. Coral holobionts exposed to the treatment for 3 months (October - December 2024)

Microbiome investigated from 16s rDNA sequencing on a NovaSeq 6000

Metabolome by untargeted metabolomics (LC-MS/MS) on a Q-Exactive Plus Orbitrap in positive ionization mode

Description of the data and file structure

Sample metadata "chap4_sampleMetadata.csv"

Script for figure compilation "figures.R"

Microbiome analysis :

Sequence table "seqtab.no1.rds"

Taxonomy table "taxa_NS_chap4.rds"

Cleaned phyloseq object "ps.filt.rds"

Script for all microbiome analysis "microbiome_analysis_clean.Rmd"

Metabolome analysis :

MS1 feature intensities "coralendosw_pos_MS1.csv"

MS1 with MS2 feature intensities "coralendosw_pos_MS2.csv"

FBMN data "coralendosw_pos_molnet.xlsx"

SIRIUS data "siriusclass_coral_pos.xlsx"

Script for all metabolomics analysis "metabolome analysis clean.Rmd"

Multiomic analysis :

Coral microbiome data clean "endo_df_corr.xlsx"

Coral metabolome data clean "msendo_corr.xlsx"

Script multiomic "coral_multiomic_clean.Rmd"

Code/Software

Scripts coded on R version 4.2.3