Transcriptomic analysis of deceptively pollinated Arum maculatum (Araceae) reveals association between terpene synthase expression in floral trap chamber and species-specific pollinator attraction

Name: Transcriptomic analysis of deceptively pollinated Arum maculatum (Araceae) reveals association between terpene synthase expression in floral trap chamber and species-specific pollinator attraction
Creator: Department of Genetics and Evolution, University of Geneva, Quai Ernest-Ansermet 30, 1211 Geneva, Switzerland; Geneva Natural History Museum, Route de Malagnou 1, 1208 Genève, Switzerland; CNRS – University of Corsica, Laboratory Sciences for the Environment, Natural Resources Project, Vignola – Route des Sanguinaires, 20000 Ajaccio, France; Université de Neuchâtel, Institut de Biologie, Rue Emile-Argand 11, 2000 Neuchâtel, Switzerland
Published: 2022-07-07T00:00:00+08:00

Zenodo2022-07-07 更新2026-04-07 收录

欺骗性传粉机制

萜烯合酶表达调控

数据链接：

https://zenodo.org/record/6806365 数据链接链接失效反馈

官方服务：

资源简介：

A compressed folder containing the R script and input files required to replicate the results in our manuscript entitled "Transcriptomic analysis of deceptively pollinated Arum maculatum (Araceae) reveals association between terpene synthase expression in floral trap chamber and species-specific pollinator attraction". Note: Raw Illumina sequencing files associated with this study have been uploaded to NCBI SRA, under the BioProject accession PRJNA856436. ABSTRACT Deceptive pollination often involves volatile organic compound (VOC) emissions that mislead insects into performing non-rewarding pollination. Among deceptively pollinated plants, Arum maculatum is particularly well-known for its potent dung-like VOC emissions and specialized floral chamber, which traps pollinators – mainly Psychoda phalaenoidesand P. grisescens – overnight. However, little is known about the genes underlying the production of many A. maculatumVOCs, and their influence on variation in pollinator attraction rates. Therefore, we performed de novo transcriptome sequencing of A. maculatum appendix and male floret tissue collected during- and post-anthesis, from ten natural populations across Europe. These RNA-seq data were paired with GC-MS analyses of floral scent composition and pollinator data collected from the same inflorescences. Differential expression analyses revealed candidate transcripts in appendix tissue linked to malodourous VOCs including indole, p-cresol, and 2-heptanone. Additionally, we found that terpene synthase expression in male floret tissue during anthesis significantly covaried with sex- and species-specific attraction of Psychoda phalaenoides and P. grisescens. Taken together, our results provide the first insights into molecular mechanisms underlying pollinator attraction patterns in A. maculatum, and highlight floral chamber sesquiterpene (e.g.bicyclogermacrene) synthases as interesting candidate genes for further study.

提供机构：

Department of Genetics and Evolution, University of Geneva, Quai Ernest-Ansermet 30, 1211 Geneva, Switzerland; Geneva Natural History Museum, Route de Malagnou 1, 1208 Genève, Switzerland; CNRS – University of Corsica, Laboratory Sciences for the Environment, Natural Resources Project, Vignola – Route des Sanguinaires, 20000 Ajaccio, France; Université de Neuchâtel, Institut de Biologie, Rue Emile-Argand 11, 2000 Neuchâtel, Switzerland

创建时间：

2022-07-07