Fig3G_RACE_luc-GCTG_R2_LG133_annotated.tif
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HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with or without 99 bp repeated sequence or an additional GCTG tetramer. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
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figshare创建时间：
2023-01-19
