The entry vectors differ only in the overhangs that are created by BsaI restriction digest. These overhangs define the position of the fragment in the final assembly. Defined amino acid linker sequenc
(A) In agreement with recent electron cryotomography experiments [30, 31], we allow for additional CheW-only linkers (yellow) connecting two TDs (black and blue). (B) Exemplary ensemble of complexes i
The entry vectors differ only in the overhangs that are created by BsaI restriction digest. These overhangs define the position of the fragment in the final assembly. Defined amino acid linker sequenc