Determination of the WWOX gene function in modulating cellular pathways activated by AP-2α and AP-2γ transcription factors in bladder cancer

The aim of the current study was to investigate how various levels of WWOX, AP-2α and AP-2γ affect signaling pathways in bladder cancer cell lines. Global transcriptome analysis using CAGE-seq was conducted. The study was based on three bladder cancer cell lines i.e. RT-112, HT-1376 and CAL-29 for which overexpression of genes encoding WWOX, AP-2α and AP-2γ was induced by lentiviral transduction. Investigated variants included: Control (treated with lentrivirus blank controls); native WWOX with AP-2α overexpression; native WWOX with AP-2γ overexpression; WWOX overexpression with native AP-2α/γ; WWOX and AP-2α overexpression; WWOX and AP-2γ overexpression.