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Demonstration that SybrGOLD staining is not necessary to quantify Salmonella O-antigen phenotypes by flow cytometry using an anti-O:5 polyclonal rabbit anti-sera (Difco) and a human monoclonal IgG specific for S.Typhimurium O:12-0. Conclusion: No difference in population fractions staining negative, single- or double-positive is observed with gating of SybrGOLD-positive event or total population Stained sample with no bacteria added is shown, identified as well E6.

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2021-04-01
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