Assessment of gene expression changes during human cortical interneuron development

We have used our protocol for generating cortical interneurons from human embryonic stem cells (hESCs) to study gene expression changes during this process, to identify regulatory networks critical to cortical interneuron development. Samples were collected at day 0 (hESCs), day 15 (ventral telencephalic patterned medial ganglionic eminence-like progenitors), day 35 (immature interneurons), and day 60 (mature interneurons). For each time point, 3-4 biological replicates generated from independant differentiations of hESCs were used for transcriptomic analysis by RNA-sequencing.