Characterizing the transcriptional role of L-DOPA and antimycin A on mitichondrial regulation in Cryptococcus neoformans using a cir1 knockout strain

Proper mitochondrial function is critical for the ability of the fungal pathogen Cryptococcus neoformans to cause disease. Mitochondrial dysfunction of WT cells treated with antimycin A or L-DOPA, or on cells lacking Cir1 is evident from this dataset. These experiments revealed influences on transcript levels of genes encoding Fe-S cluster assembly components and the response to oxidative stress in Cryptococcus neoformans. Wildtype strains were treated with and without L-DOPA or antimycin A for 6 hours after a 16 hour initial growth period in rich and minimal media respectively prior to RNA isolation. The cir1 knockout strain was also grown in rich media for 16 hours prior to RNA isolation. 3 biological replicates were used for each treatment.