Primer sequences used for RT-PCR amplification of M, H, and F genes of peste des petits ruminants (PPRV) and identification of recombinant bacmids.

The primers used for RT-PCR amplification of the M, H, and F genes of PPRV. The primer pairs M1/M2, H1/H2, and F1/F2 were used to amplify the M, H, and F genes, respectively. The underlined nucleotidesin the forward primers are a Kozak sequence added to optimize the expression of the target foreign gene. Restriction enzyme sites were introduced at the respective 5′-termini (shown in bold); the relevant enzymes are indicated in the last column. The primer pairs M13 For/M13 Rev were used to identify the recombinant bacmids.