O-glycans on the HNV G stalk modulate fusion.

A) Schematic of the HNV G protein. The dashed lines denote the O-glycan rich region in which mutants were created, with the insert showing the amino acid sequence of that region. Amino acids determined by mass spectrometry to be O-glycosylated in HeV are in bold. CT = cytoplasmic tail, TM = transmembrane. B&D) Cell surface expression (CSE, white) fusion (black), and ephrinB2 binding (grey) levels of generated HeV G (B) or NiV G (D) mutants. All values were normalized to wt HeV or NiV G. Averages and standard deviations are shown. N = 5. Statistically significant differences, as determined by a Student’s t-test, are marked with an * (p< 0.05). C, E) Western blot analysis of cell lysates from HeV G (C) or NiV G (E) mutants.