RNA profiling of cervical cancer exosomes using next- generation sequencing.

In the preset study we isolated and characterized exosomes from both HPV positive and negative cervical cancer cell lines and identified a subset of mRNA’s enriched specifically in HPV positive exosomes through deep sequencing methods. Potential target prediction gene ontology, KEGG pathway analysis revealed possible functions associated with differentially expressed genes of CaCx exosomes. Profiling of these exosomes also revealed the export of HPV 16 associated transcripts (E1,E2, E5, and L2) within SiHa exosomes, however no such export was observed for major oncogenic transcripts (E6 and E7). Our Reverse transcription PCR experiments could validate our deep sequencing results for the absence of E6, E7 transcripts in exosomes. Interestingly, PCR could amplify a truncated version of E6 transcript in HeLa exosomes, which was not detectable in our sequencing results. To study the differentially expressed genes in HPV-positive and negative cervical cancer cell lines exosomes