Competition for iron shapes metabolic antagonism between Bacillus subtilis and Pseudomonas

Siderophores have long been implicated in sociomicrobiology as determinants of bacterial interrelations. For plant-associated genera like Bacillus and Pseudomonas, siderophores are often acclaimed for their function in biocontrol. Here, we set out to determine the functional role of the Bacillus subtilis siderophore bacillibactin in an antagonistic interaction with Pseudomonas marginalis. The presence of bacillibactin strongly influences the outcome of the interaction in an iron-dependent manner. A bacillibactin producer B. subtilis restricts the colony spreading of P. marginalis, repress the transcription of histidine kinase-encoding gene gacS, and thereby abolish production of secondary metabolites such as pyoverdine and viscosin. In contrast, the lack of bacillibactin restricts B. subtilis colony growth in a mechanism reminiscent of a siderophore tug-of-war for iron. Our study identifies a Bacillus-Pseudomonas interaction conserved across fluorescent Pseudomonas spp., expanding our u..., Culturing
B. subtilis DK1042 (the naturally competent derivative of 3610) and Pseudomonas marginalis PS92 were routinely cultured in lysogeny broth (LB, Lennox, Carl Roth, 10 g/L tryptone, 5 g/L yeast extract, and 5 g/L NaCl), LB supplemented with 1% (V/V) glycerol and 0.1 mM MnCl2 (LBGM), or King’s B medium (KB; peptone 20 g/L, glycerol 1% (V/V), K2HPO4 8.1 mM, MgSO4·7H2O 6.08 mM) at 30 °C. Colonies were grown on media solidified with 1.5% agar (W/V). FeCl3, ammonium ferric citrate and 2,2’-bipyridine (BPD) were supplemented into autoclaved agar at varying concentrations. Antibiotics were added in the following final concentrations: Gentamycin (Gm) 50 μg/mL, Ampicillin (Amp) 100 μg/mL, Chloramphenicol (Cm) 5 μg/mL, and Kanamycin (Km) 10 μg/mL.
DK1042 and PS92 were routinely spotted at a 5.0 mm distance on agar surfaces using 2 µL culture at OD600 of 1.0. Prior to spotting, plates were dried for 30 minutes in a lateral flow hood and were then incubated at 30 °C. When spotting cocultures..., All image files can be opened using FIJI with Bioformats., # Title of dataset: Competition for iron shapes metabolic antagonism between Bacillus subtilis and Pseudomonas

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This dataset contains raw .czi images from the related manuscript \"Competition for iron shapes metabolic antagonism between Bacillus subtilis and Pseudomonas\" by Lyng et al. 2023.
The study uses information about bacterial colony size to determine the cause behind a mutual antagonism between B. subtilis and P. marginalis.
We routinely cultured colonies in close proximity on agar surfaces and imaged the colonies, using constitutively expressed fluorophores to identify and measure each strain.

## Description of the data and file structure

The dataset is a collection of compressed files in .zip format. You will need compression software to unpack the .zip-files.

Each .zip file contains images for an experiment from the associated manuscript.
The images relate to the manuscript figures as such:

\"base_interaction\" - Figure 1 and S1
\"complementation\" - Figure 2 and S4
\"dhb_...