Dense, systematic mutagenesis to identify the features required for CSEs
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https://www.ncbi.nlm.nih.gov/sra/SRP401528
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Investigate the sequence features required for CSE activity and selectivity. Overall design: Candidate region was synthesized as 80 190 nt oligonucleotides tiled in 5 nt steps. Each wild type 190 nt oligonucleotide was mirrored by a matching mutant in which the central 5 nt were deleted. The activity of mutant and mirrored wt oligos was compared to identify the import sequence.
创建时间:
2026-01-22



