Total flavone of Abelmoschus manihot Lmedic inhibits the metastasis of colorectal cancer cells via inhibition of the STAT3/EMT signaling pathway by up-regulating LncRNAAL137782

Total flavone of Abelmoschus manihot Lmedic (TFA) is a natural compound known for its anti-inflammatory, analgesic, and antioxidant properties. Despite extensive investigations into the therapeutic potential of TFA, there remains a paucity of reports regarding its impact on cancer treatment. This study aimed to elucidate the effects and mechanisms of TFA in CRC therapy. Subsequently, we employed RNA-seq technology to scrutinize differences in the gene expression profiles between TFA-treated and untreated CRC cells. Our findings underscore that TFA suppresses the growth, migration, and apoptosis of CRC cells. In animal models, TFA inhibits CRC cell growth and reduces hepatic metastasis. Mechanistically, we observed that TFA accomplishes this by upregulating long non-coding RNA AL137782, which, in turn, inhibits the EMT/STAT3 signaling pathway. Overall design: In order to study the inhibitory effect of TFA on the malignant phenotype of colorectal cancer cells, we used TGF-ß-induced DLD-1 cells for 24 h, and then intervened with TFA to observe the changes in cell migration and apoptosis.