HPM

The Human Proteome Map (HPM) portal is an interactive resource to the scientific community by integrating the massive peptide sequencing result from the draft map of the human proteome project. The project was based on LC-MS/MS by utilizing of high resolution and high accuracy Fourier transform mass spectrometry. All mass spectrometry data including precursors and HCD-derived fragments were acquired on the Orbitrap mass analyzers in the high-high mode. Currently, the HPM contains direct evidence of translation of a number of protein products derived from over 17,000 human genes covering >84% of the annotated protein-coding genes in humans based on >290,000 non-redundant peptide identifications of multiple organs/tissues and cell types from individuals with clinically defined healthy tissues. This includes 17 adult tissues, 6 primary hematopoietic cells and 7 fetal tissues. The HPM portal provides an interactive web resource by reorganizing the label-free quantitative proteomic data set in a simple graphical view. In addition, the portal provides selected reaction monitoring (SRM) information for all peptides identified.人类蛋白质组图谱（Human Proteome Map, HPM）数据库是面向科研共同体的交互式资源，整合了人类蛋白质组计划草图项目产生的海量肽段测序结果。该项目采用液相色谱-串联质谱（Liquid Chromatography-Tandem Mass Spectrometry, LC-MS/MS）技术，并使用高分辨率、高精度的傅里叶变换质谱仪开展实验。所有质谱数据，包括前体离子及HCD（高能碰撞解离，Higher-energy Collisional Dissociation）衍生的片段离子，均采用轨道阱（Orbitrap）质量分析器以高-高采集模式完成采集。目前，基于来自临床确认健康个体的多器官/组织及细胞类型的超过29万条非冗余肽段鉴定结果，HPM数据库已获得超过17000个人类基因编码的多种蛋白质产物的直接翻译证据，覆盖人类已注释蛋白编码基因的84%以上。该数据集包含17种成人组织、6种原代造血细胞以及7种胎儿组织。HPM数据库以简洁的可视化视图重构无标记定量蛋白质组数据集，为用户提供交互式网页资源。此外，该数据库还为所有已鉴定的肽段提供了选择反应监测（Selected Reaction Monitoring, SRM）相关信息。

搜集汇总

背景与挑战

背景概述

Human Proteome Map（HPM）是一个交互式资源，整合了人类蛋白质组项目的肽段测序结果，基于LC-MS/MS和高分辨率傅里叶变换质谱技术。它覆盖了超过17,000个人类基因（占注释蛋白编码基因的84%以上），基于超过290,000个非冗余肽段鉴定，涉及30个器官/细胞类型，包括成人组织、胎儿组织和造血细胞，并提供无标记定量数据和SRM信息。