RNA-seq Analysis of Vesicular Stomatitis Virus (VSV)-Infected RAW264.7 Macrophages with and without Prior BCG-lysate Exposure

Our findings demonstrated that BCG treatment significantly reduced the intracellular viral titers of Vesicular Stomatitis Virus (VSV). To explore the molecular mechanisms responsible for the enhanced antiviral immunity of BCG-trained macrophages, we conducted RNA sequencing analysis on RAW264.7 cells infected with VSV, comparing macrophages pre-exposed to BCG with those without prior exposure. To explore the impact of BCG-induced trained immunity on antiviral responses, we initially established an in vitro cellular model by treating RAW264.7 cells with BCG lysate for 24 hours, followed by a 5-day rest period. Subsequently, both PBS-treated and BCG-trained RAW264.7 cells were infected with Vesicular Stomatitis Virus (VSV) in vitro for 48 hours.