Lef1 Ablation Alleviates Cartilage Mineralization during Post-traumatic Osteoarthritis

Cartilage mineralization is a tightly controlled process, imperative for skeletal growth and fracture repair. However, in osteoarthritis (OA), cartilage mineralization may impact the joint range of motion, inflict pain and may increase chances for joint effusion. Here we attempt to understand the link between inflammation and cartilage mineralization by targeting SIRT1 and LEF1, both reported to have contrasting effects on cartilage. Overall design: mRNA was extracted from ribcage E17 chondrocytes bearing a Lef1 fl/fl and ATCLef1 fl/fl genotype