Metagenomic sequencing of Golden Snub-nosed Monkey's feces

Four metagenomic pools were created by equimolar pooling of qualified DNA samples:SNJ-1: Spring wild+captiveSNJ-2: Summer captiveSNJ-3: Summer wildSNJ-4: Diarrheal samples collected across both spring and summerThese qualified DNA samples were submitted to LingEn BioTech Co., Ltd. for metagenomic shotgun library construction. Sequencing was performed on the Illumina NovaSeq 6000 platform, generating 150 bp paired-end reads.Gut pathogen dynamics were profiled by aligning metagenomic contigs to the Pathogen-Host Interactions Database. In healthy monkeys, low-abundance potential pathogens were detected, including Salmonella enterica, Staphylococcus aureus, and Porphyromonas gingivalis, all below clinical relevance thresholds. No notable variations occurred among healthy groups, which abundance differences less than 20%, indicating stable colonization of commensal pathobionts across seasons and habitats.In contrast, diarrheal monkeys exhibited profound pathogen expansion: E. coli increased more than 150-fold, while Klebsiella pneumoniae emerged at 8.7%. Concurrently, Salmonella enterica increased 40-fold.