Transcriptomic changes in Arabidopsis thaliana upon the expression of mutated TFIIS

The expresion of dominant negative version of Arabidopsis TFIIS (TFIISmut) in plants lacking funtional TFIIS leads to severe growth defects. The genome-wide analysis of transcriptomic changes upon TFIISmut expression was performed by RNA-seq to better understand the biological importance of TFIIS. 10DAS seedlings of transgenic lines carring inducible GFP-TFIIS or GFP-TFIISmut transgenes were studied by RNA-seq following plants exposure to 24h b-estradiol or EtOH (mock) induction. Overall design: Arabidopsis mRNA profiles of 10-day old transgenic plants with inducible expression of GFP-TFIIS or GFP-TFIISmut fusion proteins in tfIIs-1 background were generated by deep sequencing and analysed comparatively with uninduced plants.