Peanut oral immunotherapy suppresses clonally distinct subsets of T helper cells

Food allergy affects an estimated 8% of children in the US, with increasing severity and global prevalence. Using single-cell RNA sequencing and paired TCR sequencing, we assessed the transcriptomes of CD154+ and CD137+ peanut-reactive T helper cells from 12 peanut-allergic patients longitudinally throughout peanut oral immunotherapy. These results demonstrate a differential response to OIT among subsets of peanut-reactive T helper cells, and indicate that non-Th2 activation signatures may be associated with clinical outcomes. Single-cell RNA and TCR sequencing of human samples via Seq-Well. ***Human raw data will be uploaded to dbGaP for controlled access