File S1 - Glycosylation Variants of a β-Glucosidase Secreted by a Taiwanese Fungus, Chaetomella raphigera, Exhibit Variant-Specific Catalytic and Biochemical Properties

Contains the following files: Figure S1. Supernatant of P. pastoris X33 with or without pGAPZα-D2His6. Figure S2. Size-exclusion chromatography fraction profile of YP + 20 mM glucose inoculated with spores, monitored by pNPG (closed squares) and cellobiose (open circles) assays. Figure S3. NSI-FT full mass spectrum of permethylated released O-glycans from nD2L. Figure S4. Smaller forms of D2 are more resistant to higher glucose concentrations than nD2L in pNPG assays. Figure S5. nD2S and rD2 have almost identical pH optima at pH 5.0, while nD2L is most active in lower pH. Figure S6. A time course of plate- and spore-inoculated nD2 electromobility by in-gel MUG activity assay. Table S1. MS/MS identification of native D2 variants. (DOCX)