Replication Data for: Recovery and deep sequencing of short ssDNA pools from transient, fuel-dependent coacervate droplets

<p>
Raw and processed data for the manuscript 'Recovery and deep sequencing of short ssDNA pools from transient, fuel-dependent coacervate droplets'.
</p>
<p>
Author list: Anna-Lena Holtmannspoetter*, Corbin Machatzke*, Job Boekhoven<sup>#</sup>, Hannes Mutschler<sup>#</sup>
*authors contributed equally
<sup>#</sup>co-corresponding authors
</p>

<p>
All scripts used to generate the data can be found at:
<a href="https://github.com/hmutschler/droplet-dna-analysis"> https://github.com/hmutschler/droplet-dna-analysis</a>
</p>
<p>
The data is structured in the following folders:<br>

<ul>
<li>
'raw_data', which contains the raw sequencing reads as FASTQ files (three samples and one unidentified sample, each with R1 and R2 reads),
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<li>
'cut_adap', which contains the sequencing reads after adapter trimming, in .FASTQ format (three samples, each R1 and R2 read),
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<li>
'len_fil', which contains the sequencing reads after length filtering, in .FASTQ format (three samples, each with R1 and R2 reads),
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<li>
'per_base_content', which contains the output files from analysing nucleotide content as .PDF files (7 figures),
</li>
<li>
'mfe', which contains the original 'file_list.txt' required for the analysis script, the output data from the analysis script in .txt format (three samples, four files each) and the output figure in .pdf format,
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<li>
'streme_output', which contains the output from MEME Suite's STREME software: a file containing all motif data in .txt format and output data from the analysis script in .pdf format,
</li>
<li>
'structure', which contains the output files from the RNAshapes analysis as .txt files (three samples) and the output figures from the analysis script as .pdf files (two figures).
</li>
</ul>

</p>
The same dataset was also used in the following study:<br>
Machatzke, C., Holtmannspötter, A.-L., Mutschler, H. and Boekhoven, J. (2025). DNA affects the phenotype of fuel-dependent coacervate droplets'.
https://chemrxiv.org/engage/chemrxiv/article-details/68a63016a94eede154afd83c.