Data underlying the publication: Ultrasound Transparent Neural Interfaces for Multimodal Interaction - In vivo experiments

<strong>Data sets of in vivo experiments (FUSI data of awake mice)</strong><strong>Functional ultrasound imaging sequence of in vivo experiments</strong>Similar to the phantom experiments, ultrasound images were acquired using a 18 MHz linear array (L22-14vX with 128 piezoelectric elements, 100 μm pitch, 8 mm elevation focus, Verasonics, USA) connected to a programmable ultrasound scanner (Vantage 256 High frequency configuration, Verasonics, USA). We transmitted a sequence of 12 angled plane waves(-10° to 10°), resulting in a 1 kHz framerate after compounding. Ensembles of 200 compounded frames were acquired every0.2 s for a total period of 1150 s, resulting in an effective power Doppler rate of 5 Hz. During acquisition, RF data was real time delay-and-sum beamformed to complex IQ frames, which where saved for later offline Doppler processing.<br><strong>fUS acquisition of in vivo experiments</strong>Mice were habituated to head-fixation on a running wheel in a dark environment (0- 2 lux) by progressively increasing the restraining period from 10 up to 90 minutes over 7 days. After the habituation period, an experiment session consisting of 2 recordings was conducted on two consecutive days when the animal was 13 weeks old. Mice were facing a monitor (U2410, Dell, USA) ∼20 cm in front of them. The ultrasound probe is fixed to a micromanipulator enabling adjustments to target the superior colliculus (SC). Acoustic gel (Aquasonic MAT-00-28152 ,Parker Laboratories, USA) was applied on the cranial window for ultrasound coupling before placing the ultrasound probe 2 mm above the TPX. For the first recording, functional scans were acquired without the NEP placed on the cranial window. During the second recording, the NEP was placed on the cranial window. The next experiment session was done in a reverse sequence. A recording consisted of 10 min of baselinerecording and 10 stimuli, each containing a 15-second-long visual stimulation with an interval stimulus interval (ISI) of 30 s baseline monitor display (grey screen). The visual stimuli consisted of black and white full-field drifting gratings (50 degrees per second, 30- 32 lux) moving in one of 8 different directions (angle: 0, 45, 90, 135, 180, 225, 225, 270, 315; randomized sequence) for 1 s each. Again, a ParC-based sample was used as NEP ( 2.5 μm ParC, 300 nm Au with a 50 nm Ti adhesion layer,and 1.5 μm ParC insulation)