Effect of flg22 peptide over transcriptome and nucleosome landscape of Arabidopsis on Col-0 and chromatin remodelling ATPase EDA16 mutants

Activation of plant immunity by bacterial MAMPs such as flg22 affects nucleosome positioning over thousands of loci and is correlated with the transcription of immune-related genes. Data for chromatin remodelling ATPase EDA16 truncated and over-expressor mutants as well as Col-0 (control) are provided. Overall design: 2-week-old seedlings were treated with 100 nM flg22 or mock-treated for 2 hours. Material was frozen and ground in liquid nitrogen splitting sample into 2 batches; one for RNA-seq and one for MNase-seq. Col-0, eda16-OE (SAIL_40_F09) and eda16-?Hc (SAIL_735_G06) seedlings were used. This experiment was carried out in duplicate with 24 samples in total (12 samples for RNA-seq and 12 samples MNase-seq). Samples single-end sequenced with NextSeq 500 Illumina sequencer