Sequencing two DNA templates in five channels by digital compression.
收藏PubMed Central1993-03-01 更新2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC45936/
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资源简介：
By applying algebraic coding methods to the Sanger dideoxynucleotide procedure, DNA sequences of two templates can be determined simultaneously in only five reactions and data channels. A 5:2 data compression is accomplished by instantaneous source coding of nucleotide sequence pairs into one set of 5-bit block codes. A general algebraic expression, 2n-1 > or = 4f, describes conditions under which f DNA templates can be sequenced using n channels. Such compression sequencing is accurate and efficient, as demonstrated by manual 35S autoradiographic detection and automated on-line analysis using fluorescent-labeled primers. Symmetric 5:2 compression is especially useful when comparing two closely related sequences. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1993-03-01
