RT2 Profiler Array Data for Adventitia and PVAT at 8 and 24 weeks on control and high-fat diet

The RT2 Profiler Array uses a Microsoft Excel-based file that allows for import of CT values from the real time PCR machine. Data were gathered in separate Excel sheets for each of the following four comparison: 8 week Adventitia HF vs Control; 8 week PVAT HF vs Control; 24 week Adventitia HF vs Control; and 24 week PVAT HF vs Control. The threshold value for each plate in this study was set to 0.16. In each sheet, CT values were compared to the average of five (5) different reference genes: b-actin (Actb), beta-2-microglobulin (B2M), hypoxanthine phosphoribosyltransferase 1 (Hprt1), lactate dehydrogenase A (Ldha) and Ribosomal protein, large, P1 (Rplp1). The 2-DCT were calculated for the gene of interest where DCT is [CT value of the gene of interest – C T value of averaged house keeping gene]. From this, the fold change of HF/control was calculated. These are the values reported in Supplemental Table 1. A fold change of 2.00 or above was considered biologically meaningful. Statistically, the p values were calculated based on a Student’s t-test of the replicate 2-DCT values for each gene in the Control group and Treatment (HF) groups.