iTRAQ-Based Membrane Proteomics Reveals Plasma Membrane Proteins Change During HepaRG Cell Differentiation

HepaRG cell, a stabilized bipotent
liver progenitor cell line,
exhibits hepatocyte functions only after differentiation. However,
the mechanism of transition from nondifferentiated to differentiated
states, accompanied by proliferation migration and differentiation,
remains poorly understood, particularly those proteins residing in
the plasma membrane. In this study, the membrane protein expression
change of HepaRG cell during differentiation were systematically analyzed
using an iTRAQ labeled quantitative membrane proteomics approach.
A total of 70 membrane proteins were identified to be differentially
expressed among 849 quantified membrane proteins. Function and disease
clustering analysis proved that 11 of these proteins are involved
in proliferation, migration, and differentiation. Two key factors
(MMP-14 and OCLN) were validated by qRT-PCR and Western blot. Blockade
of MMP-14 further demonstrated its important function during tumor
cell migration. The data sets have been uploaded to ProteomeXchange
with the identifier PXD004752.