The impact of inhibiting Dgat2 and Pnpla2 functional enhancers on the transcriptome of 3T3-L1 adipocytes.

In order to explore the impact of enhancer inhibition on adipocyte differentiation and triglyceride deposition at the transcriptome level, we infected cells with lentiviral vectors containing dCas9-KRAB-sgRNAs (experimental group) or dCas9-KRAB-Control (control group) at a multiplicity of infection (MOI) of 100 for 12 hours in 1 mL culture medium. Transcriptome analysis via RNA sequencing (RNA-seq) was conducted on dCas9-KRAB-Dgat2-En4, dCas9-KRAB-Pnpla2-En5, and dCas9-KRAB-Control cells at day 7 of differentiation. Overall design: Gene expression profiling analysis of mRNA-seq data from dCas9-KRAB-Dgat2-En4, dCas9-KRAB-Pnpla2-En5, and dCas9-KRAB-Control cells on the 7th day of differentiation.